
Protein is essential for muscle growth and repair. The body breaks down protein into amino acids, which are used to build muscle fibres. The harder you train, the more protein your body requires. Consuming protein after a workout is beneficial for muscle protein synthesis (MPS), which is the process of repairing damaged muscles. For muscle growth and repair, the recommended protein intake is 1.4–2.0 g of protein per kg of body weight per day.
| Characteristics | Values |
|---|---|
| What is protein the main component of | Muscles, bones, organs, skin, and nails |
| Muscle protein synthesis (MPS) | The natural process where protein is produced to repair the damage to muscles caused by intensive exercise |
| What is MPS triggered by | An acute exercise stimulus, especially resistance exercise, and protein ingestion |
| How much protein is needed after a split session | 20-25g |
| How much protein is needed after a whole-body workout | 40g |
| What happens to the protein after consumption | Enzymes in the stomach and small intestine break them down into chains of amino acids called peptides |
| What are peptides | Chains of amino acids used as bricks and mortar by the body to rebuild muscle fibres |
| What happens after the amino acids are formed | They are sent to the liver, which propels them to the muscles |
| What happens after the amino acids reach the muscles | They stimulate the growth of myofibrils, which are the filaments that make up muscles |
| What are myofibrils | Bundles of protein filaments that are critical to muscle contraction |
| What happens after the myofibrils are formed | They fuse with the damaged areas of muscle fibres, making the muscle bigger and stronger |
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What You'll Learn

Amino acids are the building blocks of protein
The shape of a protein is dictated by the sequence and location of amino acids within it. Amino acids are linked together by peptide bonds, forming polypeptides or proteins. The folded shape, or conformation, of a protein depends on the linear amino acid sequence. The body can synthesise non-essential amino acids, but essential amino acids must be supplied from an exogenous diet. Essential amino acids include histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, and valine.
Amino acids play a crucial role in repairing health and protecting the body from diseases. They can decrease protein breakdown during exercise and reduce levels of creatine kinase, an indicator of muscle damage. By incorporating foods rich in amino acids, individuals can improve their mood, sleep, athletic performance, and muscle gain. Additionally, amino acids stimulate muscle protein synthesis (MPS), which is the driving force behind adaptive responses to exercise.
For physically active individuals, whole food sources of protein that contain all the essential amino acids are recommended as they stimulate MPS. Pre-sleep casein protein intake can increase overnight MPS and metabolic rate without influencing lipolysis. Exercise also has an anabolic effect that lasts at least 24 hours, working synergistically with protein ingestion when consumed before or after resistance exercise.
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Muscle repair and growth
Exercise plays a crucial role in muscle repair and growth. Strength training is particularly effective in building muscle, while cardiovascular activity also provides benefits. The best results are achieved when exercise is combined with adequate rest. Regular cardio can improve muscle growth and function while boosting overall fitness levels. However, insufficient rest can hinder muscle repair and slow fitness progress. It is important to allow each muscle group to rest adequately and avoid consecutive days of strength training for the same muscle group.
Diet is another key factor in muscle repair and growth. Consuming adequate amounts of protein is essential. The current Recommended Dietary Allowance (RDA) for protein is defined as the minimum amount required to prevent lean body mass loss. However, higher-protein diets have been associated with potential muscle-related benefits. Whole food sources of protein that contain essential amino acids can stimulate muscle protein synthesis (MPS), which is crucial for building and maintaining muscle mass. The amount of protein required depends on the intensity and type of exercise performed. Enzymes in the stomach and small intestine break down protein into amino acids, which are then transported to the liver and subsequently to the muscles.
Age, sex, and genetics also influence muscle development. For example, males typically experience faster muscle growth due to factors such as larger muscle mass, higher testosterone levels, and tighter joints. Additionally, older adults may require a two-phase approach to mitigate the loss of muscle mass and function, including dietary education and habitual exercise.
Supplementation can also play a role in muscle repair and growth. For instance, glutamine supplementation has been found to reduce muscle soreness and attenuate the inflammatory response after exercise. Coenzyme Q10 supplementation has been suggested to offer anti-inflammatory benefits and aid in recovery. Furthermore, crocodile blood supplementation has been studied for its potential effects on muscle-damaging exercise, indicating positive results in maintaining peak muscle force and reducing muscle soreness.
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Protein-rich foods
Consuming protein-rich foods is essential for maintaining optimal health during normal growth and ageing. Protein-rich foods provide the body with the necessary building blocks to maintain and repair muscle tissue.
Plant-based sources of protein include legumes, such as lentils, chickpeas, and black beans, which are excellent choices for vegetarians and vegans. They are not only rich in protein but also provide other essential nutrients like fibre, folate, potassium, and iron. Additionally, nuts and seeds, including almonds, walnuts, peanuts, and chia seeds, offer a good balance of protein, healthy fats, vitamins, and minerals.
It is recommended to include a variety of protein-rich foods in your diet to ensure sufficient intake of essential amino acids and other nutrients. For those who avoid animal-based proteins, combining different plant-based sources throughout the day can provide a healthy mix of amino acids.
The amount of protein required varies depending on individual needs and activity levels. Physically active individuals, including athletes, may require higher amounts of protein and can benefit from supplementation to ensure adequate intake without consuming too many calories. However, it is important to note that excessive protein intake, especially from red meat or processed meat, has been linked to increased risks of cardiovascular disease and diabetes. Therefore, a balanced approach that includes a variety of protein sources is key to maintaining overall health and supporting muscle health.
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Timing of protein intake
While the overall daily protein intake is the most important factor in facilitating muscle growth, timing protein intake strategically can also help to enhance muscle protein synthesis, support muscle recovery, and promote optimal muscle growth.
Protein timing is a common practice among athletes, bodybuilders, and individuals aiming to build muscle. It involves consuming protein at specific times of the day to maximize benefits such as increased muscle mass. During the night, muscle protein synthesis decreases, and your body remains in a catabolic state, breaking down muscle protein. Therefore, consuming a protein-rich meal before bed can help mitigate this. Ensuring a steady supply of protein throughout the night can aid in muscle recovery and growth.
Consuming protein shortly before and after resistance exercise can significantly enhance muscle protein synthesis. This practice is known as the "anabolic window of opportunity", a period when your body is best positioned to absorb and use nutrients. While the science is not entirely conclusive, many athletes and bodybuilders find it beneficial to time their protein intake around their workouts.
For those with specific fitness goals, such as muscle building or fat loss, consuming protein at strategic times—such as in the morning, post-workout, or after fasting—can offer additional benefits. Ultimately, understanding and applying the principles of protein timing can help maximize the effectiveness of your nutrition strategy, but it should always be tailored to individual needs and overall dietary patterns.
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Recommended dietary allowance (RDA)
The Recommended Dietary Allowance (RDA) for protein is defined as the minimum amount required to prevent lean body mass loss. It is often misrepresented and misinterpreted as a recommended optimal intake. The current RDA for protein is 0.8 grams of protein per kilogram of body weight, or 0.36 grams per pound. This is the amount of the nutrient one needs to meet their basic nutritional requirements. It is not the specific amount one is supposed to eat every day. The RDA is the minimum amount required to keep one from getting sick.
The World Health Organization (WHO) has established international adult protein recommendations of 0.83 grams per kilogram of body weight per day, which is similar to the current RDA. However, the RDA has been criticized for being too low and not accounting for the varied needs of certain groups. For instance, older adults are 40% less efficient than young adults at producing muscle protein from dietary protein. Similarly, pregnant and lactating women, as well as children over three years old, have higher protein requirements.
The protein requirements of several different populations have been evaluated using the IAAO methodology, which has resulted in recommendations that are at least double and nearly triple the RDA for strength and endurance athletes. Researchers have recommended boosting the RDA of protein for older adults to about 1.25 grams per kilogram of body weight per day. For children over three years old, they suggest increasing it to 1.55. For pregnant or lactating women, they propose hiking it to between 1.6 and 2, depending on the stage of pregnancy and nursing.
The current RDA for protein may not be sufficient for physically active individuals who require higher protein intake to support muscle growth and repair. The relationship between diet and protein balance is complex, and protein intake alone may not be enough to stimulate muscle protein synthesis (MPS) effectively. Exercise, particularly resistance exercise, plays a crucial role in muscle growth and works in synergy with protein ingestion. For building and maintaining muscle mass, a daily protein intake in the range of 1.4–2.0 grams of protein per kilogram of body weight is recommended for people exercising.
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Frequently asked questions
Protein is a nutrient that provides the body with a structural framework. It is made up of amino acids that join together to form long chains. It is the main component of muscles, bones, organs, skin, and nails.
After consuming protein, enzymes in the stomach and small intestine break them down into chains of amino acids called peptides. These amino acids are sent to damaged muscle cells to stimulate the growth of myofibrils, which are the filaments that make up muscles.
Timing is crucial when it comes to consuming protein. It is generally considered that consuming protein shortly after exercise is beneficial for muscle protein synthesis (MPS). The body breaks down protein faster immediately following exercise, and also synthesizes it more actively, leading to more amino acids being absorbed into the muscles.
The amount of protein one should consume depends on various factors such as age, gender, and physical activity level. The current Recommended Dietary Allowance (RDA) for protein is defined as the minimum amount required to prevent lean body mass loss. For American adults, the RDA is 0.83 g/kg/d. However, for building and maintaining muscle mass, a daily protein intake in the range of 1.4-2.0 g/kg/d is recommended for people exercising.











































