
A muscle bundle, also known as a fasciculus, is a group of muscle fibres surrounded by a layer of connective tissue called the perimysium. Each muscle fibre is a single cylindrical muscle cell, and each bundle can contain hundreds or thousands of muscle fibres. Muscle fibres are also known as myofibers or myofibrils, and when bundled together, they form a striated pattern of sarcomeres, which are the fundamental contractile unit of a skeletal muscle.
| Characteristics | Values |
|---|---|
| Definition | A group of muscle fibres ensheathed by connective tissue (perimysium) |
| Muscle fibres | Cylindrical muscle cells |
| Muscle fascicles | Each bundle of muscle fibre is called a fasciculus |
| Connective tissue | The outermost sheath of connective tissue covering each muscle is called epimysium |
| Connective tissue layers | 3 layers: epimysium, perimysium, and endomysium |
| Muscle contraction | Occurs when a sarcomere shortens, causing the Z-discs to move closer together and the I band to become smaller |
| Muscle movement | Controlled by the nervous system |
| Muscle bundles and meat grain | Finer-grained meats are more tender and have smaller bundles, while coarser-grained meats are tougher and have larger bundles |
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What You'll Learn

Muscle bundles are groups of muscle fibres
Muscle bundles, also known as fascicles, are groups of muscle fibres surrounded by a layer of connective tissue called the perimysium. Each muscle fibre is a single cylindrical muscle cell, and each bundle can contain thousands of these fibres. The connective tissue covering provides support and protection for the muscle fibres, allowing them to withstand the forces of contraction.
Skeletal muscles are made up of muscle bundles, and each bundle is wrapped in perimysium. The perimysium is a connective tissue sheath that separates the bundles and provides structure to the muscle. Between the muscle bundles, we find clusters of fat cells, small blood vessels (capillaries), and nerve branches. The connective tissue sheath outside the perimysium is called the epimysium, and it surrounds the entire muscle. Fascia, another type of connective tissue, is found outside the epimysium and separates the muscles from one another.
The perimysium and epimysium play a crucial role in muscle contraction. The epimysium, along with the tendon or aponeurosis, attaches the muscle to the periosteum of bones. When the muscle contracts, the tension is transferred through the connective tissue layers to the tendon, and then to the periosteum, resulting in the movement of the skeleton. Before a skeletal muscle fibre can contract, it must receive an impulse from a nerve cell. This impulse causes a rapid depolarization of the motor endplate, initiating an action potential in the muscle fibre, leading to contraction.
The structure of muscle bundles and their arrangement within skeletal muscles vary. Skeletal muscles can range from extremely fine strands to large masses, and the size, shape, and fibre arrangement differ between muscles. The bundles themselves are composed of smaller cylindrical muscle fibres of varying lengths. These fibres are arranged in a unique striated pattern, forming sarcomeres, which are the fundamental contractile units of skeletal muscles.
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They are surrounded by a layer of connective tissue called the perimysium
A muscle bundle, or fasciculus, is a group of muscle fibres bundled together. Each bundle of muscle fibres is surrounded by a layer of connective tissue called the perimysium. The perimysium is also called interfascicular connective tissue. It is a sheath of dense irregular connective tissue that groups muscle fibres into bundles. The perimysium is made up of type I and type III collagen, with some sources also citing the presence of type VI and type XII collagen. The collagen fibres are wavy and change shape when the muscle lengthens or shortens.
The perimysium is an extension of the epimysium, which is a connective tissue layer that surrounds individual skeletal muscles. The epimysium projects inward to divide the muscle into compartments, with each compartment containing a bundle of muscle fibres. The perimysium separates the muscle bundles within the skeletal muscle and provides support and protection for the muscle cells. It also contains a network of blood vessels and nerves, known as neurovascular bundles, that supply the muscle fibres with nutrients and oxygen.
The perimysium is involved in transmitting contractile movements and forces generated in the fascicles. During muscle contraction, the perimysium transmits the force produced by individual muscle fibres, generating smooth and coordinated muscle movements. The perimysium, along with the epimysium and endomysium (connective tissue surrounding individual muscle cells), extend beyond the fleshy part of the muscle to form a tendon or aponeurosis, which attaches the muscle to bones or other muscles.
The size of muscle bundles varies, with finer-grained meats having smaller bundles and coarser-grained meats having larger bundles. Muscle bundles are further divided into smaller cylindrical muscle fibres of varying lengths.
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Skeletal muscle fibres are long and cylindrical
A muscle bundle, or fasciculus, is a bundle of muscle fibres surrounded by a layer of connective tissue called the perimysium. Muscle fibres are long, multinucleated cells that are commonly referred to as muscle fibres or myofibers due to their length and cylindrical shape. Skeletal muscle fibres can be quite large compared to other cells, with diameters up to 100 μm and lengths up to 30 cm in the Sartorius of the upper leg.
Skeletal muscle fibres are composed of myofibrils, which are made up of sarcomeres linked in series. The striations of skeletal muscle are created by the organisation of actin and myosin filaments, resulting in the banding pattern of myofibrils. These actin and myosin filaments slide over each other to cause the shortening of sarcomeres and the cells to produce force. The specialised smooth endoplasmic reticulum, called the sarcoplasmic reticulum (SR), stores, releases, and retrieves calcium ions (Ca++).
The plasma membrane of muscle fibres is called the sarcolemma, and the cytoplasm is referred to as the sarcoplasm. Skeletal muscle fibres can contain a large number of nuclei, which allow for the production of large amounts of proteins and enzymes needed to maintain normal function. In addition to nuclei, skeletal muscle fibres also contain cellular organelles found in other cells, such as mitochondria.
Skeletal muscles vary considerably in size, shape, and arrangement of fibres. They range from extremely tiny strands such as the stapedius muscle of the middle ear to large masses such as the muscles of the thigh. Skeletal muscles have an abundant supply of blood vessels and nerves, which is directly related to their primary function of contraction. Before a skeletal muscle fibre can contract, it must receive an impulse from a nerve cell.
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Muscle fibres are bundled together and wrapped in connective tissue
Skeletal muscles are considered organs of the muscular system. Each skeletal muscle may be made up of hundreds or even thousands of muscle fibres bundled together and wrapped in a connective tissue covering. Each muscle fibre is a single cylindrical muscle cell.
These bundles of muscle fibres are called fascicles and are surrounded by a layer of connective tissue called the perimysium. Within each fascicle, each muscle fibre is encased in a thin connective tissue layer of collagen and reticular fibres called the endomysium.
The connective tissue covering provides support and protection for the delicate muscle fibres, allowing them to withstand the forces of contraction. It also provides pathways for the passage of blood vessels and nerves. The epimysium, perimysium, and endomysium often extend beyond the fleshy part of the muscle to form a tendon or a broad, flat sheet-like aponeurosis.
This fascicular organization is common in the muscles of the limbs. It allows the nervous system to trigger specific movements by activating a subset of muscle fibres within a bundle. In skeletal muscles that work with tendons to pull on bones, the collagen in the three connective tissue layers intertwines with the collagen of a tendon, which then fuses with the periosteum coating the bone.
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Muscle fascicles are useful diagnostic tools for dermatomyositis
A muscle bundle, or fasciculus, is a bundle of skeletal muscle fibres surrounded by a layer of connective tissue called the perimysium. Each bundle of muscle fibres is known as a muscle fascicle. Muscle fascicles are useful diagnostic tools for dermatomyositis, a rare condition that causes muscle inflammation and is characterised by muscle weakness and skin rash.
Dermatomyositis is an idiopathic inflammatory myopathy that affects the skin and muscles and, in rare cases, the joints, oesophagus, and lungs. It is strongly associated with malignancy, especially in adults. The disease presents with characteristic skin findings and symmetric proximal skeletal muscle weakness. The muscle fascicle structure is a useful diagnostic tool for dermatomyositis, as the myocytes towards the edges of the muscle fascicle are typically narrower, while those at the centre are of normal thickness.
The histopathological features of dermatomyositis include perifascicular atrophy, which is the atrophy of muscle fibres, especially around the periphery of fascicles. This is a hallmark feature of the disease. Additionally, injury to intramuscular blood vessels can occur in the form of immunoglobulin and complement deposits on endomysial capillaries, leading to reduced capillary density and endothelial hyperplasia.
Initial testing for dermatomyositis includes muscle enzyme levels, such as creatine kinase and lactate dehydrogenase. Further diagnostic studies may include imaging to screen for underlying malignancy, such as CT scanning and ultrasound. Muscle biopsy can also be performed, and findings of perivascular and interfascicular inflammatory infiltrates with muscle fibre degeneration and regeneration can be diagnostic for dermatomyositis.
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Frequently asked questions
A muscle bundle is a group of muscle fibres surrounded by connective tissue known as perimysium. Each bundle of muscle fibre is called a fasciculus.
Inside a muscle bundle are muscle fibres, also known as muscle cells. These fibres are long and cylindrical and are commonly referred to as myofibers.
Muscle bundles allow the nervous system to trigger a specific movement of a muscle by activating a subset of muscle fibres within a fascicle of the muscle.











































